Comparative Modulation of the Reproductive System by Ethanolleaf Extracts of Asystasia Gangetica Andanthocleista Vogelli in Male Wistar Rats



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Submitted 2020-05-17
Published 2020-05-30
10.24018/ejmed.2020.2.3.287

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A number of medicinal plants have been found to influence the level of reproductive hormones and thus affect fertility in the overall. This work evaluated the effects of ethanol leaf extracts of A. gangetica and A. vogelli respectively on some reproductive system parameters in male Wistar rats. A total of sixteen (16) male albino rats were grouped into four (four rats in each group): group A served as normal control, group B received Immunace (Vitabiotics) group C and D received 400 mg/kg body weight of A. gangetica and A. vogelli extracts respectively. Extracts were administered orally to rats for 21 days, after which they were sacrificed by cervical dislocations and blood samples drawn by cardiac puncture. The effect of the extracts on testosterone, follicle stimulating hormone, luteinizing hormone and semen analysis of the test rats were determined using enzyme linked immunosorbent assay (ELISA) and standard techniques. Data collected were analyzed using Graph pad prism V6 and p values < 0.05 were adopted as significant. There was no significant (p>0.05) change in testosterone, FSH and luteinizing hormones in the group administered A. gangetica plant extract relative to the control. There was also no visible difference in the testes weight and sperm morphology relative to the control group. In contrast, administration of A. vogelli extract caused significant (p<0.05) decrease in testosterone and follicle stimulating hormone concentrations from: 1.12 ± 0.20 to 0.89 ± 0.05 and 1.41 ± 0.07 to 1.35 ± 0.12 respectively relative to the control group. While significant (p<0.05) decrease in luteinizing hormone (1.71 ± 0.15) was observed relative to the standard drug group (1.76 ± 0.05). A significant (p<0.05) decrease in sperm count and testes weight was also observed in rats treated with A. vogelli extract relative to the normal control.The results suggest that, A. vogelli extract may cause decreased fertility in male albino rats and could be developed further into potent male contraceptives. A. gangetica on the other hand, had no effect on male reproductive hormones.

Keywords: Anthocleistavogelii, Asystasia gangetica, modulatory, reproductive

References

  1. F. Ghaffari, and A. Arabipoor. The role of conception type in the definition of primaryand secondary infertility. Int J Reprod Biomed (Yazd), vol. 16, no. 5, pp. 355–356, 2018.
    DOI  |   Google Scholar
  2. A.A. Dada and V.O Ajilore. Use of ethanol extracts of Garcinia kola as fertility enhancerin female catfish Clariasgariepinusbroodstock. International Journal Fish and Aquaculture., vol. 1, no. 1, pp. 005–010, 2009.
     Google Scholar
  3. M. Ganguly, M. Borthakur, N. Developmenti, R. Mahanta. Antifertility activity of the methanolic leaf extract of Cissampelospareira in female albino mice. J. Ethnopharmacol., vol. 111, pp. 688–691, 2007.
    DOI  |   Google Scholar
  4. L. K. Merlin, G. K. Mensah, D. F. Arnold, F. Caleb, K. A. Alexande and A. D. Rita. Toxicity and Safety Implications of Herbal Medicines Used in Africa, Herbal Medicine, Philip F. Builders, IntechOpen, 2019
    DOI  |   Google Scholar
  5. M. M. Burns. Alternative medicine: Herbal preparation. Clinic Emergency Medicals, vol. 1, pp. 186-190, 2000.
    DOI  |   Google Scholar
  6. N. Desai, R. Sharma, K. Makker and E.A.A Sabannegh. Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men. Fertilizer Sterilization, vol. 92, pp. 1626-1631, 2009.
    DOI  |   Google Scholar
  7. J. E. Otoide. Preliminary Inventory of the Vegatation at the proposed site of the Ekiti State University Botanical Garden. World News of Natural Sciences. vol. 24, pp. 36-53, 2019.
     Google Scholar
  8. O. S. Adedeji, G. O. Farimi, S. A. Ameen and J. B. Olayemi. Effects of bitter kola (Garcinia kola) as growth promoter in Broiler Chicks fromday old to four weeks old. Journal Animal Veterinary, vol. 5, no. 3, pp. 191–193, 2009.
     Google Scholar
  9. G. K. Anjana, S. Dilip, K. Sandeep and K. B. Ashok. Immune modulating property of Ocimum sanctum by regulating the IL-2 production and its mRNA expression using rat’s splenocytes. Asian Pacific Journal Tropical Medication, vol. 3, no. 1, pp. 8–12, 2010.
    DOI  |   Google Scholar
  10. S. H. Kotta, A. Shahid, and J. Ali. Exploring scientifically proven herbal aphrodisiacs. Pharmacog Rev, vol. 7, no. 13, pp. 1–10, 2013.
    DOI  |   Google Scholar
  11. P. S. Shajeela, V. R. Mohan, J. L. Louis and S. P. Tresina. Antifertility acitivity of ethanol extract of Dioscorea esculenta (L.) Schott on male albino rats. Int J PharmTech Res, vol. 3, no. 2, pp. 946-954, 2011.
     Google Scholar
  12. S. O. Oladimeji, J. O. Igbalaye and C. L. Coleshowers. Immunological biomarkers determined in female rats administered with pro-fertility extract ofAnthocleistavogelliJournal National Science Resource, vol. 4, pp. 113–123, 2014.
     Google Scholar
  13. O. A. Esimai, E. O. Orji and A. R. Lasisi. Male contribution to infertility in IIe-Ife, Nigeria. Nigeria Journal of Medicine, vol. 11, no. 2, pp. 70-72, 2002
     Google Scholar
  14. I. A. Jegede, J. A. Ibrahim and O. F. Kunle. Phytochemical and pharmacognostic studies of the leaf and stem-bark of Anthocleista vogelli (Planch). Journal of Medicinal Plants Research,vol.5, no. 26, pp. 6136-6139, 2011.
    DOI  |   Google Scholar
  15. G. O. Anyanwu, E. C. Onyeneke, U. Usunobun and A. J. Adegbegi. Impact ofAnthocleistavogelliroot bark ethanolic extract on weight reduction in high carbohydrate diet induced obesity in male wistar rats. African Journal of Biochemistry Research, vol. 7, no. 11, pp. 225–232, 2013.
    DOI  |   Google Scholar
  16. E.G. Nieschlag, F. Weinbauer, T. G. Cooper, W. Wittkowski and T. Cantz. Reproduktion. In: Speckmann E-J, Hescheler J, Köhling R (eds.) Physiologie 5. Auflage, Urban & Fischer München pp. 652–677, 2008.
     Google Scholar
  17. G. F. Weinbauer, C. M. Luetjens, M. Simoni, and E. Nieschlag. Physiology of Testicular Function. In: Nieschlag, E., Behre, H.M., Nieschlag, S. (edition). Andrology-Male Reproductive Health and Dysfunction 3rdeddition . Verlag Berlin Heidelberg : Springer pp.11-60, 2010
    DOI  |   Google Scholar
  18. S. R. Babu, M. D. Sadhnani, M. Swarna, P. Padmavathi and P. P. Reddy. Evaluation of FSH, LH and testosterone levels in different subgroups of infertile males. Indian J Clin Biochem, vol. 19, no. 1, pp. 45-9, 2004.
    DOI  |   Google Scholar
  19. N. N. Tietz. (Ed). Clinical Guide to Laboratory Tests. 3rd Ed. Philadelphia: W.B Saunders Pp 216-217, 1995.
     Google Scholar
  20. M. E. Uotila and E. Ruoslahti. Radioassay System in Clin. J. Immunol. Methods, vol. 42, pp. 11, 1981.
    DOI  |   Google Scholar
  21. Y. E. Ameyaw, S. Duker, D. George and S. D. Mills-Robertson. Assessment of Variation in Some Medicinal Plant Species. Ethno biological Leaflets, vol. 1, no. 12, pp. 1-6, 2008.
     Google Scholar
  22. J. O. Moody, V. A. Robert, J. D. Connolly and P. J. Houghton. Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of SphenocentrumjollyanumPierre (Menispermaceae),J.Ethnopharmacol. vol. 104, pp. 87-91, 2006
    DOI  |   Google Scholar
  23. N. E. Amidu, K. B. A. Woode, A. Owiredu, A. K. William,
     Google Scholar
  24. C. George-Boateng. An Evaluation of Toxicity and Mutagenicity of Sphenocentrumjollyanum. International Journal Pharmacology 4: 67-77, 2008.
    DOI  |   Google Scholar
  25. M. M. Iwu. Handbook of African medicinal Plants, CRC Press.BocaRatot, Pp. 241-251, 1993.
     Google Scholar
  26. M. N. Ushijima, Y. Komoto, I. Sugizono, M. Mizuno, M. Sumihiro, M. Ichikawa and M. Hayama. Triterpene glycosides from the root of Codonopsislanceolata,Biol.Pharm.Bull, vol. 56, no. 3, pp. 308-314, 2008.
    DOI  |   Google Scholar
  27. P. J. O’Shaughnessy, A. Monteiro, G. Verhoeven, K. De Gent and M. H. Abel. Effects of FSH on Testicular Morphology and Spermatogenesis in Gonadotrophin-Induced Hypogonadal Mice lacking androgen receptors. The Journal of society for Reproduction and Fertility, vol. 139, no. 1, pp. 177-184, 2010.
    DOI  |   Google Scholar
  28. E. V. Ikpeme, P. B. Udoh, U. B. Ekaluo, O. Udensi and B.O Asuquo. Spermicidal and Hormonal Response of Wistar Rats Treated with Ethanol Seed, Leaf and Pulp Extracts of Carica Papaya (Linn). International Journal of Recent Scientific Research, vol. 7, 155-159, 2010.
     Google Scholar
  29. P. B. Ayoola, O. O. Onawumi and O. O. Faboya. Chemical evaluation and nutritive values of Tetracarpidiumconophorum (African walnut) Edition. Journal of Pharmaceutical and Bio-medical Science, vol. 11, pp. 1-4, 2011.
     Google Scholar
  30. K. O. Oyedeji, A. F. Bolarinwa and A. M. Akintola. Effect of Methanol Extract of Vernonia amygdalina on Haematological and Plasma Biochemical Parameters in Male Albino Rtas.Journal of Dental and Medical Sciences vol. 3, pp. 64-67, 2013.
    DOI  |   Google Scholar
  31. S. Jain, G. P. Choudhary and D. K. Jain. Medicinal plants with potential anti-fertility activity: A review. International of Green Pharmacy, vol. 9, no. 4, pp. 223-228, 2015.
     Google Scholar