Administration of Recombinant Platelet Derived Growth Factor-Bb (PDGF-Bb) Intrafracture on Shaft Femur Rat Wistar Performed Osteotomy and Intramedullary Wire Improves Healing Process of Fractures, Collagen Type I And III Levels



Abstract Views
399

Downloads
306

Citations

Share

##plugins.themes.bootstrap3.article.sidebar##

Submitted Jul 11, 2022
Published Sep 5, 2022
10.24018/ejmed.2022.4.5.1420

Abstract viewed = 399 times

Table of Contents

##plugins.themes.bootstrap3.article.main##

Introduction: Of all the fractures that can occur in human body, one of the most lethal is femoral fracture. Treatment of femoral fracture can be divided into two, which is operative and non-operative. External fixation, intramedullary nailing, locked intramedullary nail, and plate and screw are the options for operative treatment. Platelet Derived Growth Factor (PDGF-BB) have essential role of wound healing, due to positive effect of upregulating proliferation of fibroblast, synthesize of extracellular matrix, and revascularization. In this study we aimed to see the effect of PDGF-BB fracture healing process, type I and III collagen levels in the Wistar rats femoral shaft treated with osteotomy and intramedullary nailing.

Methods: This study is an experimental randomized post-control only to animal with femoral shaft osteotomy and intramedullary wire installation in Wistar Rats that added PDGF-BB intrafracture, to the collected data then analyzed using Pearson test in SPSS 25.

Results: Based on the independent t test result, we found that callus formation, type I and III collagen expression on the femur shaft of wistar rats that has been administered PDGF-BB intrafracture had a statistically significant result compared to those who had not been administered PDGF-BB intrafracture with P<0.05.

Conclusion: Mean Allen score, type I collagen and type III collagen levels of shaft femur in Wistar rats group that been osteotomized and attached by intramedullary wire added with PDGF-BB is higher compared to non PDGF-BB.

Keywords: Allen score, femoral fracture, PDGF-BB, type I collagen, type III collagen

Downloads

Download data is not yet available.

References

  1. Mattisson L, Bojan A, Enocson A. Epidemiology treatment and mortality of trochanteric and subtrochanteric hip fractures: data from the Swedish fracture register. BMC Musculoskeletal Disorders. 2018; 19(369): 1-8.
    DOI  |   Google Scholar
  2. Hussain A, Adham JE. Intramedullary Kirschner wire (K-wire ) fixation of femoral fracture in children. J Child Orthop. 2007; 1(5): 277–80.
    DOI  |   Google Scholar
  3. Sahu RI. Percutaneous K. Wire fixation for femur shaft fractures in children: A treatment concepts for developing countries. Ann Med Health Sci Res. 2013; 3(2): 197-201.
    DOI  |   Google Scholar
  4. Antariksa IMI. Bone Healing in femoral fracture of white rat with intramedullary wire fixation and additional medullary bone marrow. Journal of Indonesian Orthopaedic. 2012; 40: 13–6.
     Google Scholar
  5. Ekegren CL, Edwards ER, de Steiger R, Gabbe BJ. Incidence, costs and predictors of non-union, delayed union and mal-union following long bone fracture. International Journal of Environmental Research and Public Health. 2018; 15(12): 1-11.
    DOI  |   Google Scholar
  6. Solchaga LA, Hee CK, Roach S, Snel LB. Safety of recombinant human platelet-derived growth factor-BB in Augment® Bone Graft. J Tissue Eng. 2012; 3(1): 2041731412442668.
    DOI  |   Google Scholar
  7. Yoshimoto T, Yamamoto M, Kadomatsu H, Sakoda K, Yonamine Y, Izumi Y, et al. Recombinant human growth/differentiation factor-5 (rhGDF-5) induced bone formation in murine calvariae. Journal of Periodontal Research. 2006; 41: 140-7.
    DOI  |   Google Scholar
  8. Graham S, Leonidou A, Lester M. Investigating the role of PDGF as a potential drug therapy in bone formation and fracture healing. Expert Opinion on Investigational Drugs. 2009; 18: 1633–54.
    DOI  |   Google Scholar
  9. Rodrigues M, Griffith GL, Wells A. Growth factor regulation of proliferation and survival of multipotential stromal cells. Stem Cell Research & Therapy. 2010; 1(32): 1-12.
    DOI  |   Google Scholar
  10. Caplan AI, Correa D. PDGF in bone formation and regeneration: New insights into a novel mechanism involving MSCs. Journal of Orthopaedic Research. 2011; 29: 1795–803.
    DOI  |   Google Scholar
  11. Hanaoka K, Tanaka E, Takata T, Miyauchi M, Aoyama J, Kawai N, et al. Platelet-derived Growth Factor Enhances Proliferation and Matrix Synthesis of Temporomandibular Joint Disc-derived Cells. Angle Orthod. 2006; 76(3): 486-92.
     Google Scholar
  12. Ojima Y, Mizuno M, Kuboki Y, Komori T. In vitro effect of platelet-derived growth factor-BB on collagen synthesis and proliferation of human periodontal ligament cells. Oral Diseases. 2003; 9(3): 144-51.
    DOI  |   Google Scholar


Most read articles by the same author(s)